ATP结合元件A1与肾间质泡沫细胞形成、小管间质损害及血清胆固醇的相关性

中华肾脏病杂志 ›› 2011, Vol. 27 ›› Issue (11) : 819-824.

临床研究

苏华付玲刘建社朱红艳

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Correlations of ABCA1 with foam cells formation in renal interstitium, tubulointerstitial lesion and serum cholesterol

SU Hua, FU Ling, LIU Jian-she, ZHU Hong-yan.

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目的研究肾小球肾病患者肾间质区域ATP结合元件A1（ABCA1）蛋白的表达及其与泡沫细胞形成、肾小管间质损害、血清胆固醇的相关性。方法选取诊断明确的Alport综合征患者5例、膜增生性肾小球肾炎（MPGN）患者28例、局灶节段性肾小球硬化（FSGS）患者35例、特发性膜性肾病（IMN）患者36例、IgA肾病（IgAN）患者34例为研究对象。采用免疫组织化学法检测患者肾间质区域（除肾小管）ABCA1蛋白的表达；用直线相关分析其与肾间质区域泡沫细胞相对面积、肾小管间质损害积分、血清胆固醇的相关性。结果在各类肾小球疾病患者的肾间质区域中，浸润的单核巨噬细胞、泡沫细胞及肾小管上皮细胞均表达ABCA1蛋白。泡沫细胞浸润者肾间质区域ABCA1蛋白的阳性表达量显著高于无泡沫细胞浸润者（P < 0.05），但ABCA1的阳性表达量与泡沫细胞相对面积无相关。MPGN、FSGS、IMN患者肾间质区域ABCA1表达量与肾小管间质损害积分呈正相关（P < 0.05）。血清胆固醇与肾间质区域ABCA1蛋白的阳性表达量呈正相关（P < 0.05）。结论在各种病理类型的肾小球疾病患者中，血清胆固醇可促进肾间质ABCA1的表达；ABCA1对胞内脂质的转运具有双向作用，其与泡沫细胞形成缺乏肯定关联；ABCA1可能参与了肾小管间质的损害。

Abstract

Objective To investigate the correlations of ATP-binding cassette transporter A1 (ABCA1) protein expression in renal interstitium with foam cells formation, tubulointerstitial lesion and serum cholesterol in glomerulonephropathy. Methods Five patients with Alport syndrome, 28 patients with membranous proliferative glomerular nephritis (MPGN), 35 patients with focal segmental glomerulosclerosis (FSGS), 36 patients with idiopathic membranous nephropathy (IMN) and 34 patients with IgA nephropathy (IgAN) were enrolled in the study. Expression of ABCA1 protein was detected by immunohistochemical method. The relative area of foam cells in renal interstitium was semi-quantified and the correlations of ABCA1 protein expression in renal interstitium (except for renal tubules) with the relative area of foam cells, integration of tubulointerstitial lesion (TIL) and serum cholesterol were examined using t test and linear correlation analysis. Results In renal interstitium, monocyte-macrophages, foam cells and renal tubular epithelial cells expressed ABCA1 protein. ABCA1 protein expression in renal interstitium with foam cells infiltration was significantly higher as compared to that without foam cells infiltration (P< 0.05), but there was no correlation between ABCA1 expression and foam cells area (P>0.05). In MPGN, FSGS, IMN patients, there was a positive correlation between ABCA1 protein expression in renal interstitium and integration of TIL (P<0.05). There was a positive correlation between serum cholesterol level and ABCA1 protein expression in renal interstitium (P<0.05). Conclusions In glomerulonephropathy, cholesterol may promote the ABCA1 expression in renal interstitium. ABCA1 may have double-effects on transportation of cholesterol. ABCA1 may take part in the process of tubulointerstitial lesion.

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苏华付玲刘建社朱红艳. ATP结合元件A1与肾间质泡沫细胞形成、小管间质损害及血清胆固醇的相关性[J]. 中华肾脏病杂志, 2011, 27(11): 819-824.

SU Hua;FU Ling;LIU Jian-she;ZHU Hong-yan. . Correlations of ABCA1 with foam cells formation in renal interstitium, tubulointerstitial lesion and serum cholesterol[J]. Chinese Journal of Nephrology, 2011, 27(11): 819-824.

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Received	Revised	Published
1900-01-01	1900-01-01	2011-11-15
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2011-11-15

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