Objective To evaluate the protein permeability of different high-flux dialysis membranes. Methods The filtrate was sampled from the dialysate compartment of different dialysers during the routine dialysis therapy. SDS-PAGE analysis was performed with silver staining method according to the modification of Melzer and consecutive laser densitometry. Before sampling all the dialysis mode was changed to pure ultrafiltration. Results The protein pattern of filtrate from dialysis membranes was similar to that of the glomerular membrane containing IgG, transferrin, albumin, retinol binding protein and beta-2-microglobulin. Comparing different membranes there were considerable differences depending on cut-off, charge and adsorption capacity of the particular membrane. In all membranes, permeability of proteins decreased during one treatment session. Conclusion Protein permeability of high-flux dialysis membranes is similar to the glomerular membrane but modified according to pore-size, surface charge, adsorption and time on dialysis. In contrast to the glomerular membrane, in each of the investigated membranes protein permeability decreases during the session.
XU Xiao-qi;Siegfried Stiller;Helmut Mann;QIAN Jia-qi;Heinrich Melzer.
Study on the protein permeability in high-flux dialysis membranes[J]. Chinese Journal of Nephrology, 2005, 21(9): 548-551.
