目的 探讨多囊蛋白1氨基端肽(PC-1NTP)对常染色体显性多囊肾病(ADPKD)肾囊肿衬里上皮细胞增殖、细胞周期和凋亡的影响&#65377;方法 用噻唑蓝(MTT)法检测PC-1NTP对ADPKD囊肿衬里上皮细胞增殖的影响&#65377;用流式细胞仪分析细胞周期与凋亡状况&#65377;用荧光定量PCR检测细胞中细胞周期素cyclinD1、p21WAF1、 bax、bcl-2 和小染色体维护蛋白2(MCM-2)的mRNA表达&#65377;结果 PC-1NTP使ADPKD囊肿衬里上皮细胞G0/G1期细胞百分比显著增多，S期细胞百分比显著减少，并明显抑制其增殖和凋亡；同时细胞中p21和bcl-2 mRNA表达明显增高(P < 0.01),cyclinD1、bax 和MCM-2的mRNA表达明显减弱(P < 0.01或P < 0.05)&#65377;结论 PC-1NTP能明显抑制ADPKD囊肿衬里上皮细胞的增殖和凋亡，减慢细胞周期进程，其作用机制可能是通过调节G1/S关卡调节因子cyclinD1/p21WAF1和凋亡调节蛋白bcl-2/bax的表达实现的&#65377;PC-1NTP有希望成为治疗ADPKD的可选药物&#65377;

Objective To investigate the effects of polycystin-1 N-terminal peptide (PC-1NTP) on proliferation, cell cycle and apoptosis of cystic-lining epithelial cells in human autosomal dominant polycystic kidney disease (ADPKD). Methods Cystic-lining epithelial cells were treated with PC-1NTP in vitro. MTT assay was used to detect the PC-1NTP effects on cells proliferation. Cell cycle and apoptosis were analyzed by flow cytometry. The mRNA expression of cyclinD1, p21^WAF1, bax, bcl-2 and MCM-2 were measured by fluorescence quantitative PCR. Results The growth and apoptosis of cystic-lining epithelial cells were significantly inhibited by PC-1NTP. The percentage of cells in G0/G1 phase increased, while decreased in S phase remarkably. The mRNA expression of p21^WAF1 and bcl-2 was significantly higher than that in the control group(P < 0.01), while the mRNA expression of cyclinD1,bax and MCM-2 was significantly lower than that in the control group(P < 0.01 or P < 0.05). Conclusions PC-1NTP resulted in G0/G1 phase arrest and elicited an attenuating effect on the proliferation and apoptosis of cystic-lining epithelial cells, which may be realized through regulating the expression of G1/S checkpoint regulation factor cyclinD1/p21^WAF1 and apoptosis regulating protein bcl-2/bax. PC-1NTP may be a new prospective maneuver in the treatment of ADPKD.