Objective To evaluate the effects of angiotensin Ⅱ(AngⅡ)infusion on renal c-Abl expression in vivo, and on podocyte c-Abl expression change in cultured mouse podocytes. Methods Twenty four male Sprague-Dawley rats(Group C, D, E and F) were assigned to receive Ang Ⅱ (400 ng•kg-1•min-1) by osmotic minipump and of which 12 rats(Group D and F) were assigned to receive telmisartan (3 mg•kg-1•d-1), six rats received normal saline(Group B), and six rats were used as normal contro1 (Group A). Animals were sacrificed at day 14(Group C and D), day 28(Group E and F) respectively. Conditionally immortalized mouse podocytes were used in vitro. Podocytes were studied 2 weeks after thermoswitching from 33℃ to 37℃. Cells were fetal bovine serum (FBS) starved for at least 12 hours prior to stimulation. The cultured podocytes were treated with Ang Ⅱ doses ranging from 10-9 mol/L to 10-6 mol/L and for different hours. Expression of renal and podocytes c-Abl was examined by immunofluorescence staining, real-time PCR and Western blotting. Results (1) Distribution of c-Abl expression was mainly in the cytoplasm and nuclear of the podocytes in vivo and in vitro. (2) Expressions of c-Abl mRNA and protein were increased in AngⅡ-infused rat podocytes and AngⅡ-induced cultured mouse podocytes (P＜0.05), and the effects of AngⅡ were dose-dependent and time-dependent in vitro. Conclusion There are c-Abl mRNA and protein expression in podocytes, and c-Abl may play a critical role in the pathogenesis of AngⅡ-induced podocyte injury.
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