Objective    To examine whether aldosterone contribute to obesity related glomerular disease.    Methods    C57BL/6J mice were randomly divided into three groups: a control group (low-fat-diet, n=10), a model group (high-fat-diet, n=10) and a intervention group (high-fat-diet, n=12). After 8 weeks intervention group were treated with a mineralocorticoid receptor antagonist, spirolactone (SPL).The physicochemical indexes and the renal pathology of the three groups were all detected. The mRNA and protein expressions of podocyte marker protein were determined by real-time PCR and Western blotting, respectively.    Results    Compared with the control group, body weight, kidney weight, Lee’s index, fat index, blood cholesterol, blood triglyceride, creatinine clearance rate, urinary protein excretion, glomerular average diameter, glomerular foot process average width were significantly up-regulated (P＜0.05); The mRNA and protein expression of nephrin, podocin, podoplanin and podocalyxin were significantly down-regulated in model group (P＜0.05). Meanwhile, these changes were attenuated by SPL.    Conclusion    Aldosterone can participate in the process of obesity- related renal injury, and these can be attenuated by mineralocorticoid receptor antagonist, spirolactone. This gives us preliminary clues to treat ORG.